小鼠纖維肉瘤細(xì)胞系

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產(chǎn)品名稱: 小鼠纖維肉瘤細(xì)胞系
產(chǎn)品型號: CRL-1751
產(chǎn)品廠商: 美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔: 無相關(guān)文檔

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CRL-1751 WEHI 164 小鼠纖維肉瘤細(xì)胞系，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！

小鼠纖維肉瘤細(xì)胞系的詳細(xì)介紹

CRL-1751 WEHI 164 小鼠纖維肉瘤細(xì)胞系

ATCC^® Number:

CRL-1751?

Price:

$355.00

Designations:	WEHI 164
Depositors:	TW LeBien
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus (mouse)
Morphology:	fibroblast
Source:	Disease: fibrosarcoma Strain: BALB/c Cell Type: methylcholanthrene induced
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments:	This line was originally established by M. Rollinghoff and N.L. Warner from a fibrosarcoma induced by subcutaneous injections of 3-methylcholanthrene. After pretreatment with actinomycin D, the cells are highly sensitive to human cytotoxic monocytes, to human tumor necrosis factor and to lymphotoxin. They can be used as target cells for short term chromium release cytotoxicity assays. Tested and found negative for ectromelia virus (mousepox).
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:20 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes. Remove trypsin and place the flask at 37C until the cells detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020
References:	1760: Ziegler-Heitbrock HW, Riethmuller G. A rapid assay for cytotoxicity of unstimulated human monocytes. J. Natl. Cancer Inst. 72: 23-29, 1984. PubMed: 6582301 1761: Ziegler-Heitbrock HW, et al. Killer cell activity of human monoblastic leukemia cells as detected with a monocyte-specific target cell. Blood 65: 8-14, 1985. PubMed: 3155490 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 23194: Rollinghoff M, Warner NL. Specificity of in vivo tumor rejection assessed by mixing immune spleen cells with target and unrelated tumor cells. Proc. Soc. Exp. Biol. Med. 144: 813-818, 1973. PubMed: 4543620 32697: Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902

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小鼠纖維肉瘤細(xì)胞系

CRL-1751 WEHI 164 小鼠纖維肉瘤細(xì)胞系，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！

CRL-1751 WEHI 164 小鼠纖維肉瘤細(xì)胞系，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！