產(chǎn)品[
SV-HUC-1 人膀胱上皮永生化細(xì)胞
]資料
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產(chǎn)品名稱(chēng):
SV-HUC-1 人膀胱上皮永生化細(xì)胞
產(chǎn)品型號(hào):
CRL-9520
產(chǎn)品廠(chǎng)商:
美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無(wú)相關(guān)文檔
簡(jiǎn)單介紹
CRL-9520 SV-HUC-1 人膀胱上皮永生化細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！
SV-HUC-1 人膀胱上皮永生化細(xì)胞
的詳細(xì)介紹
CRL-9520 SV-HUC-1 人膀胱上皮永生化細(xì)胞
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CRL-9520?
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$417.00
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SV-HUC-1
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Wisconsin Alumni Res. Fndn.
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Biosafety Level:
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2 [CELLS CONTAIN ADENOVIRUS ]
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: ureter
Tissue: uroepithelium
Cell Type: epithelial SV40 immortalizedSV40 transformed
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uroepithelial keratins; SV40 T antigen
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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No
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The chromosome number distribution is bimodal with 50% of the cells near diploid (mode = 44 chromosomes) and 50% tetraploid (mode = 88). Both populations consistently showed seven marker chromosomes: 5p+, del(6)(p11), 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.
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11 years
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male
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: 1:2 to 1:5
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
derivative:ATCC CRL-9519
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3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990
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