產(chǎn)品[
RF/6A 猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞
]資料
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產(chǎn)品名稱:
RF/6A 猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞
產(chǎn)品型號(hào):
CRL-1780
產(chǎn)品廠商:
美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無相關(guān)文檔
簡單介紹
CRL-1780 RF/6A 猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！
RF/6A 猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞
的詳細(xì)介紹
CRL-1780 RF/6A 猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞
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CRL-1780?
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RF/6A
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F Hu
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Macaca mulatta (monkey, rhesus)
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epithelial
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Tissue: choroid; retina
Disease: normal
Cell Type: endothelial
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factor VIII; fibronectin
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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fetus
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly
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Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
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22315: Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454
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